What have you done differently to achieve a successful pregnancy with a frozen embryo transfer?

[Katiluna]

Hi all,

It's been a rough week and I feel rubbish since last Friday when we got our negative pregnancy results on our donor egg fresh transfer.

The fresh embryo transfer was done on 5th November. It was a good-quality grade 1 / day 5 embryo (but not PGT-A tested) and a medicated protocol was used. I am taking treatment for high NK cells, and Clexane 0.40ml to reduce the risk of blood clotting. I had intralipid one week befor the transfer. On the day of the transfer, the endometrial thickness was 9.1mm (tri-laminar) and progesterone was 58.

I'm 42 and I previously had three miscarriages (all conceived naturally), still no children. I had a hysteroscopy done in February this year as polyps were suspected on an ultrasound scan. Ultimately, the hysteroscopy did not find any polyps or fibroids and it showed a clear, normal uterine cavity.

Our donor is 29 and we have used ZyMot Fertile Chip to select the best sperm as my partner has sperm DNA fragmentation issues. Three good-quality blastocysts were created in this cycle using ICSI (two grade 1 embryos and one grade 2 embryo). We also used EmbryoGlue which obviously didn't help. One embryo was transferred on 5th November and now we are left with two frosties ( day 5 & day 6) and I'm not sure what we can do to increase our chances of success.

- What have you and your clinic done differently to achieve a successful pregnancy with a frozen embryo transfer?

- What protocol worked best for you?

- If you had vaginal microbiome looked into, what screening tests have you done (apart from EMMA/ALICE) and what treatment have you taken?

- How many transfers have you had until you achieved successful pregnancy with donor eggs?

I feel stuck and lost right now as I don't know how we can move forward from here and what can be done to achieve a better outcome. I understand this is a numbers game, but I can't stop thinking that there has to be a reason for every failure. I feel like we need to try something different or do more before we proceed with the frozen embryo transfer.

Any thoughts or advice would be much appreciated.

Many thanks