Biopsy and Bloods were done to investigate hair loss. Dermatologist explained the ANA came back positive at 1/320 but the ENA and ANCA rheumatoid factor was negative. He prescribed Hydroxychloroquine 200mg daily and explained my hair will not grow back. My GP informed me l was positive for Lupus so prescribed anti depressant Citalapram. While taking Hydroxy for over 3 months all my other symptoms of muscle pain, fever at night, stiffness, brain fog and chronic fatigue all which effected my quality of life and work, these symptoms have improved greatly and l feel quite well (is the wolf just sleeping), just occasionally get sever lower back pain if l stand upright too long. Looking back l have not felt as well for at least the last 5 years or so.....because ANA is positive and others negative is it Lupus or not??? Do all tests need to be positive ?