Fascinating that the researchers here even thought to use vitamin C, vitamin D3, hydrocortisone and, wait for it, T3, to produce fibres that can be used for wound healing. Starts to read as if they have been reading here on HU TUK! Where else do you see that selection being discussed, albeit not in quite the same way.
Bioconjug Chem. 2015 Jun 16. [Epub ahead of print]
Simultaneous delivery of highly diverse bioactive compounds from blend electrospun fibers for skin wound healing.
Peh P, Lim NS, Blocki A, Chee SM, Park HC, Liao S, Chan C, Raghunath M.
Blend emulsion electrospinning is widely perceived to destroy the bioactivity of proteins, and a blend emulsion of water soluble and non-soluble molecules is believed to be thermodynamically unstable to electrospin smoothly. Here we demonstrate a method to retain the bioactivity of disparate fragile biomolecules when electrospun. Using bovine serum albumin as a carrier protein; water soluble vitamin C, fat soluble vitamin D3, steroid hormone hydrocortisone, peptide hormone insulin, thyroid hormone triiodothyronine (T3), and peptide epidermal growth factor (EGF) were simultaneously blend-spun into PLGA-collagen nanofibers. Upon release, vitamin C maintained the ability to facilitate collagen I secretion by fibroblasts, EGF stimulated skin fibroblast proliferation and insulin potentiated adipogenic differentiation. Transgenic cell reporter assays confirmed the bioactivity of vitamin D3, T3 and hydrocortisone. These factors concertedly increased keratinocyte and fibroblast proliferation while maintaining keratinocyte basal state. This method presents an elegant solution to simultaneously deliver disparate bioactive biomolecules for wound healing applications.
PMID: 26079091 [PubMed - as supplied by publisher]
For those, like me, who do not know what electrospinning is: