Both NF-κB and c-Jun/AP-1 involved in anti-β2GPI/β2GPI-induced tissue factor expression in monocytes.
Our previous data has demonstrated that Toll-like receptor 4 (TLR4) and its signalling pathway can contribute to anti-β2-glycoprotein I/β2-glycoprotein I (anti-β2GPI/β2GPI) -induced tissue factor (TF) expression in human blood monocytes and acute monocytic leukaemia cell line THP-1. However, its downstream nuclear transcription factors have not been well explored. In the current study, we further investigated whether nuclear factor kappa B (NF-κB) and activator protein (AP-1) were activated and their roles in anti-β2GPI/β2GPI complex stimulating TF expression. The results showed that treatment of the cells with anti-β2GPI (10μg/ml)/β2GPI (100 mg/ml) complex could markedly increase the levels of phosphorylated NF-κB (p-NF-κB p65) and c-Jun/AP-1 (p-c-Jun), as well as TF expression. Both NF-κB inhibitor PDTC (20 μM) and AP-1 inhibitor curcumin (25 mM) could attenuate TF expression induced by anti-β2GPI/β2GPI or APS-IgG/β2GPI complex. Combination of any two inhibitors of MAPKs (SB203580/U0126 or SB203580/SP600125 or U0126/SP600125) could decrease activation of NF-κB. SB203580/SP600125 or U0126/SP600125, but not SB203580/U0126, could reduce the phosphorylation of c-Jun/AP-1. Neither NF-κB nor c-Jun/AP-1 activation caused by anti-β2GPI/β2GPI complex could be affected by TLR4 inhibitor TAK-242. In conclusion, our results indicate that both NF-κB and c-Jun/AP-1 can be activated and play important roles in the process of anti-β2GPI/β2GPI-induced TF expression in monocytes, thereby contributing to the pathological processes of antiphospholipid syndrome.
The take away here is that of the two observed pathways that promote APA antibodies, the study was using curcumin as an inhibitor for one of them. "Both NF-κB inhibitor PDTC (20 μM) and AP-1 inhibitor curcumin (25 mM) could attenuate TF expression induced by anti-β2GPI/β2GPI or APS-IgG/β2GPI complex. "
Add that to the list. Ok, so far we have vitamin D, and curcumin. I'll start another post that focuses on a hughes autoimmine supplement protocol.
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drob32
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So - is it possible that my drinking hot milk with tumeri/curcumin before bed time a few times a week could have caused my antibody blood work to turn (as one hematologist descrived it) "boring?"
I'm not advocating anyone take anything without the advice of a doctor.
But, I am advocating that we come up with a responsible list of supplements backed by peer reviewed studies that can help our condition and then present them to the doctor to make sure they are ok to take.
However it would be a good idea to have the doc run 25 hydroxy and 1,25 hydroxy (inactive and active D) to see what your values are to be even more accurate.
I'm not sure about calcium yet.
There are hundreds of medical articles which support "beneficial / wonderful effects" of some medications and other substances which are completely different IN REALITY where people are getting dreadful side effects . I know lots of people take curcumin but I have never heard of someone who completely bounced back as a result of curcumin alone. There are also articles which also suggest curcumin being detrimental in CNS Lupus patients. I don't think it's well researched though I'm utterly shocked to find that there are hundreds of curcumin-related articles.
I personally prefer to read people's opinion re. treatments such as IVIG or Plasma exchange. lol sorry I cannot be too positive on "alternative medicine" these days..
I will say that mouse models can't always be that accurate, and everyone should be careful.
Also, while that study focused on lupus, mine focused specifically on APS.
I'm not the first person to propose curcumin. Infact, a functional doctor of mine proposed it's use along with resveratrol, fish oil, and vitamin D.
He wasn't the first either. One of the most renowned functional medical doctors, dr. kharrazian has had great success with autoimmune diseases while using it.
Clearly everyones biochemistry is different and you will have to listen to your body.
I know what you mean. I realise this is a fairly light-hearted thread.
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Hughes Syndrome
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Hughes Syndrome Study
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