Ok, it's from City of Hope, I am sure they are a great cancer institution, but I did not like the hype created around AOH1996 (where are the phase 1 results that had to be published before the end of September??)...but hopefully this research can give us some help, considering the fact that (at least in Italy) Agaricus bisporus mushroom are simply the cheapest you can buy.
"The researchers profiled blood draws from some of the men participating in City of Hope’s phase 2 clinical trial. The men were under active surveillance as they took white button mushroom supplements. Focusing on eight participants’ samples before and after three months of white button mushroom treatment, the scientists found that there were less tumor-creating MDSCs and more anti-tumor T and natural killer cells, suggesting white button mushroom rebuilds anti-cancer immune defense and slows cancer growth."
This is how it was extracted, excerpt from two different studies by the same team
"Mushroom and chemical reagents
WBM extract was prepared via hot water extraction. Briefly, 6 g of freeze-dried WBM powder generated from 60 g fresh mushrooms were boiled in 1 L hot water for 3 h. The broth was centrifuged at 3000 × g for 30 min, twice, to collect the fraction of the supernatant. The liquid fraction was rotor-evaporated to dryness and then re-dissolved in 1 mL of hot water to produce a 6× mushroom extract (6 mg/µL)3. Enza, an AR antagonist, was purchased from Selleckchem (MDV3100, Selleckchem, Houston, TX). LT, a shiitake mushroom-derived immunomodulatory β-(1, 3)-glucan, was purchased from Carbosynth (FL33321, Compton, Berkshire, UK)."
"White button mushroom (WBM) extract was prepared via hot water extraction, as previously described.14-16 Briefly, 6 g of freeze-dried WBM powder, prepared from 60 g of fresh mushrooms purchased from a local market, was boiled in 1 L hot water for 3 h. The broth was centrifuged twice at 3000 × g for 30 min to collect the supernatant. The liquid fraction was rotor-evaporated to dryness and then re-dissolved in 1 mL of hot water to produce a 6× mushroom extract, containing 6 g of dried WBM powder/mL (6 g/mL). The extract was then used for both in vitro and in vivo mouse studies. Lentinan (purified β-glucan from Shiitake mushrooms) was purchased from Carbosynth (Compton, Berkshire, UK) and used as a reference botanical drug. The cytotoxic and biological activities of WBM extract or Lentinan were evaluated with either PCa cells or immune cells. Antibodies and chemicals used in this study are listed in Tables S3 and S4."
But from what I read, in the humans part of the study they used 14 grams of tablets per person per day. But I don't understand if it's extract or dried matter.
On the other hand, preliminary studies say that the effect is not dose dependent (on mice)...so I would say, let's add 100/200g of raw mushrooms per day to our diet (or most days)! In fact they did not push to find the maximum tolerated dosage for that reason.
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BLUE SKY NEWS - AUGUST 2022