Narirutin found in lemons, oranges, passion fruit, bergamot and grapefruit may be chemopreventive agent for hormone-resistant PC

[Graham49]

Narirutin. A flavonoid found in citrus fruits modulates cell cycle phases and inhibits the proliferation of hormone-refractory prostate cancer cells by targeting hyaluronidase

Author links open overlay panelShilpi Singh a b, Akhilesh Kumar Maurya c, Abha Meena a b, Nidhi Mishra c, Suaib Luqman a b

doi.org/10.1016/j.fct.2023....

Abstract

Narirutin is a dietary flavanone found in lemons, oranges, passion fruit, bergamot and grapefruit. It possesses anti-allergic, cardioprotective, neuroprotective, hepatoprotective potential, and its enriched fraction suppresses the growth of prostate cancer cells; however, there is currently no information on the chemopreventive potential of narirutin alone against hormone-refractory prostate cancer cells (PC-3) and its mode of action. Thus, the chemopreventive possibility of narirutin was investigated in PC-3 cells by utilising cytotoxicity assays. Further, a mechanism was deduced targeting hyaluronidase, an early-stage diagnosis marker, by cell-free, cell-based and in silico studies. The results indicate that narirutin reduced the viability of PC-3 cells with the inhibitory concentration range of 66.87–59.80 μM. It induced G0/G1 phase arrest with a fold change of 1.12. Besides, it increased the generation of reactive oxygen species (ROS) with a fold change of 1.34 at 100 μM. Narirutin inhibited hyaluronidase's activity in cell-free (11.17 μM) and cell-based assays (67.23 μM) and showed a strong binding interaction with hyaluronidase. Finally, the MD simulation analysis supported the idea that narirutin binding enhanced compactness and stability and created a stable complex with hyaluronidase. In addition, ADMET prediction indicates that it is a non-toxic, non-CYPs inhibitor and thus didn't alter the metabolism. The results reveal that narirutin may be a potential chemopreventive agent for hormone-resistant prostate cancer cells in addition to offering data for supporting diet-based nutraceutical agents to prevent prostate cancer.

[RJAMSG]

Thanks for sharing!

[cfhny]

Quite interesting. Thanks for posting.

[j-o-h-n]

Shilpi Singh a b, Akhilesh Kumar Maurya c, Abha Meena a b, Nidhi Mishra c, Suaib Luqman a b

Cricket team that plays in Flushing Meadows Corona Park, Queens NY.

Good Luck, Good Health and Good Humor.

j-o-h-n Wednesday 08/09/2023 6:05 PM DST