Abstract 3714: The antitumor activity of cannabis sativa and CBD in prostate cancer PC3 cells

[NewPotatoCaboose]

Abstract 3714: The antitumor activity of cannabis sativa and CBD in prostate cancer PC3 cells

aacrjournals.org/cancerres/...

Abstract

Prostate cancer is the second most frequently occurring carcinoma in males worldwide and one of the leading causes of death in men around the world. Recent studies estimate that over 1.4 million males are diagnosed with prostate cancer on an annual basis, with approximately 375 000 succumbing to the disease annually. With current treatments continuing to show severe side effects, there is a need for new treatments. In this study we looked at the effect of cannabis sativa extract, cannabidiol and cisplatin on prostate cancer cells, PC3.

Methods: In addressing the above questions, we employed the MTT assay to measure the antiproliferative effect on PC3 cells following treatment with varying concentrations of Cannabis sativa extract, cisplatin and cannabidiol. xCELLigence was also used to confirm the IC50 activity in which cells were grown in a 16 well plate coated with gold and monitor cell. Caspase 3/7 activity was also measured using 96 well-plate following treatment. Western-blot and qRT-PCR was also used to measure the gene expression of tumor suppressor genes, p53, Bax and Bcl2. Animal studies were employed to measure the growth of PC3-mouse derived cancer to evaluate the effect of compounds in vivo.

Results: From the treatment with varying concentrations of Cannabis sativa extract, cannabidiol and cisplatin, we have observed that the three compounds induced antiproliferation of PC3 cancer cell lines through the activation of caspase 3/7 activity. We also observed induction of apoptosis in these cells following silencing of retinoblastoma binding protein 6 (RBBP6), with upregulation of p53 and bax mRNA expression, and a reduction in Bcl2 gene expression. The growth of tumors in the mouse models were reduced following treatment with cisplatin and cannabidiol.

Conclusion: We demonstrated that cannabidiol is a viable therapy to treat prostate cancer cells, in combination with silencing of RBBP6. This suggests that cannabidiol rather Cannabis sativa extract may play an important role in reducing cancer progression.

Citation Format: Lesetja Raymond Motadi, Nonhlanhla B. Moleya. The antitumor activity of cannabis sativa and CBD in prostate cancer PC3 cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3714.

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I believe in high Thc oil and canaby products helping fight pc . If nothing else ,helping symptoms . Upon dx 3-2015 I started the oil . I went into imrt Lupron and Tak -700 . Then into a undetech mode right after imrt . I like indica over sativa ..it has more of a calming sedative effect on me . I have No plan to stop this natural method! 🍀🙏

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I did the RSO ( high Thc oil) as advised by Rick Simpson of 90 grams in 60 days twice in a row during and after imrt ..

[SteveTheJ]

Of course I don't know it has made a difference but 80mg 2x daily of full spectrum CBD oil is my thing. Doesn't get you high (RSO is debilitating isn't it?) and has no effect whatsoever that I can tell on my mood or ability to relax.

2 articles I was sent:

ncbi.nlm.nih.gov/pubmed/170...

herb.co/news/health/cannabi...

[NewPotatoCaboose in reply to SteveTheJ]

If it's full spectrum it will have some THC in it.

[SteveTheJ]

In TX the legal limit is 0.3%

[CAMPSOUPS in reply to SteveTheJ]

For perspective in a truly legal state limit is about 35% which actually is about all the higher no pun intended that a plant can produce.

[Chugach]

excellent- gonna go burn one down !!!

[rbtbrsrd]

When all the compounds of the cannabis plant are taken in the gentlest manner the body will pick what it needs and disregard the rest, Raphael Mechoulam