Calcitriol enhances anti prostate cancer effects for ginsenoside found in panax ginseng. In-vitro study.

[Graham49]

1α,25-Dihydroxyvitamin D3 synergistically enhances anticancer effects of ginsenoside Rh2 in human prostate cancer cells

Author links open overlay panelMohamedBen-EltrikiabSubrataDebcEmma S. TomlinsonGunsa

doi.org/10.1016/j.jsbmb.202... rights and content

Highlights

•1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and ginsenoside Rh2 have anticancer effects as a single agent.

•The combination of 1,25(OH)2D3 and Rh2 synergistically decreases cell viability and proliferation.

•1,25(OH)2D3 and Rh2 differentially regulate androgen receptor and vitamin D receptor.

•Potential synergistic mechanisms are demonstrated by 1,25(OH)2D3 and Rh2 through modulation of apoptotic proteins.

•Anticancer effects of 1,25(OH)2D3 may be achieved at a lower condcentration when combined with Rh2.

Abstract

1α,25-dihydroxyvitamin D3 (1,25(OH)2D3, commonly known as calcitriol), the most active metabolite of vitamin D3, and ginsenoside Rh2 can regulate cellular differentiation and proliferation proteins. The purpose of the present study was to assess the effect of 1,25(OH)2D3 on the anticancer activities of Rh2 in human prostate cancer cells such as androgen-dependent LNCaP and androgen-independent C4-2 in vitro. The effects of treatment with 1,25(OH)2D3 or Rh2, either alone or in combination, on prostate cancer cells were evaluated through tetrazolium-based cell viability assay, BrdU cell proliferation rate estimation assay, and Western blot protein expression analyses of nuclear receptors (androgen receptor and vitamin D receptors) and apoptotic proteins (Bcl-2, Bax, and Caspase 3). The Combination Indices (CI) and Dose Reduction Indices (DRI) of 1,25(OH)2D3 and Rh2 were calculated to determine synergistic anticancer activity using Calcusyn software (Biosoft, Cambridge, UK). The cell viability assay data indicate that Rh2 treatment alone inhibited cell viability in a concentration-dependent manner and the addition of 10 nM 1,25(OH)2D3 to Rh2 significantly enhanced its ability to reduce cell viability up to 80 % in both the cell lines. Similarly, addition of 10 nM 1,25(OH)2D3 to Rh2 significantly lowered its IC50 values for cell proliferation from the range of 32–65 μM to 14-8 μM in LNCaP and C4-2 cells. In addition, protein expression analyses indicated that the combined treatment with Rh2 and 1,25(OH)2D3 led to greater downregulation of androgen receptor expression compared to single agent exposure. Similarly, the presence of 1,25(OH)2D3 synergistically increased the pro-apoptotic actions of Rh2 in both the cell lines. Overall, 1,25(OH)2D3 augments the Rh2-mediated anticancer effects through stimulating apoptosis and reduced cell proliferation which suggests that synergism of this combination may lead to potential lower need of the active vitamin D3 and limited toxicity from it.

[Cooolone]

Link doesn't work...

And most of these in-vitro studies fall apart when introduced to the human body. Interesting all the same. D3 and Ginseng (root) are in my dietary consumption, but not for PCa. If I benefit from it otherwise then, Yippy! Lol

[Graham49]

doi.org/10.1016/j.jsbmb.2021

[RVALuna in reply to Graham49]

Graham 49, I have been calcitriol and Panax genseng for about 12 years for PCa ever since reading of researchby Beers(?) on 8 men with PCa using calcitriol of which 7 lowerd their PSA. The men did not have a problem of Hi blood calcium with .5 mg/d of Calcitriol but did on 2 pils of the .5mg pild/day. I titrated my self and found I could only handle one .5 mg /d which put me at the max normal calcium in my blood. Hi calcium is dangerous for the heart. I also take vitamin mega K2 (LEF.org) to keep calcium from my blood vessels. I also take high dose vitamin D3 for my 25 OH vitamin D3 level is at 100 ng/ml in the blood.

28 year survivor with bone and lymph node remission on casodex monotherapy after 3 1/2 years. best pedro luna

[GeorgeGlass in reply to RVALuna]

how much D3 per day? How much K2 do you use daily? Just comparing with the 5k and 180mcg that I take. Why calcitriol and not just D3+K2? Is red korean ginseng the same as panax ginseng?

[Graham49 in reply to GeorgeGlass]

I take D3 and K2.

[GeorgeGlass]

which of these are you taking? I take D3, K2 and red korean panseng