The in-vitro evaluation of anticancer effect of DMU-212, anovel resveratrol analog, on prostate cancer cells

[puxi]

B. Ozmen Yelken1

, C. Kayabaşi

1

, A. Aşik1

, T. Balci Okcanoğlu2

,

F. Soğutlu1

, R. Gasimli1

, S. Yilmaz Susluer1

, C. Biray Avci1

, C.

Gunduz1

1

Ege University Medical School, Department of Medical

Biology, İzmir, Turkey, 2

Near East University, Vocational

School of Health Sciences, Nicosia, Cyprus

Prostate cancer (PCa) is the second leading cause of cancer

among men in developed countries. It is known that Epithelial Mesenchymal Transition (EMT) plays a crucial role

in the progression and metastasis of PCa. It has been

reported that DMU-212, which is one of the resveratrol

analogs, induces G2/M arrest. In our study, we focused on

the effect of DMU-212 on the cell cycle, apoptosis,

migration, and invasion of prostate cancer cells. The cytotoxicity effect of DMU-212 on LNCaP and PC-3 cells was

determined by WST-8 test. While the apoptotic effect of

determined IC50 dosages of DMU-212 was tested by

Annexin V, PI analysis and flow cytometry were used to

evaluate its effect on cell cycle. The invasion and migration

458 J. del Picchia

of PCa cells were analyzed by “Cell Biolabs CytoSelect 96

well Cell Invasion Assay Kit” and “Wound Healing Assay”,

respectively. PCR Arrays were assessed 84 EMT-related

gene. E-cadherin and vimentin level changes were assessed

by immunofluorescence. Additionally, the protein expressions of cyclin B1, E-cadherin, and β-catenin were tested by

western blot. It was observed that the IC50 dosage of DMU212 induced apoptosis, reduced the invasion and migration,

and induced predominantly G2/M arrest in LNCaP and PC3 cells. It was shown that DMU-212 inhibited the EMT by

suppressing the WNT signaling pathway, especially, on PC3 cells. In conclusion, compatible with the hypothesis of

that the inhibition of EMT may prevent metastasis in PCa; it

is believed that the DMU-212 has a potential role in the

treatment of cancer.

B. Ozmen Yelken: None. C. Kayabaşi: None. A. Aşik:

None. T. Balci Okcanoğlu: None. F. Soğutlu: None. R.

Gasimli: None. S. Yilmaz Susluer: None. C. Biray Avci:

None. C. Gunduz: None.

[NPfisherman]

I believe that like curcumin....this could be beneficial....part of any issue with supplements is getting a high enough level into the system and maintaining it for a period of time. There has been work done on this issue with other supplements like curcumin--patch and topical cream...and to get a high enough level to be therapeutic...

Thanks for posting,

Fish

[podsart]

Is this the entire reference or can you provide link to original clinical article?

[puxi in reply to podsart]

this is a copy&paste of the original POSTER. bests