Longevity Breakthrough: New Protein Discovery Could Be the Key to Healthier Aging

[LAJ12345]

scitechdaily.com/longevity-...

pnas.org/doi/10.1073/pnas.2...

[ZebraDoodle]

”New research found that the protein MANF helps cells manage toxic protein clumps, improving cellular health and potentially aiding treatments for age-related diseases like Alzheimer’s and Parkinson’s”

[Boscoejean]

"To obtain MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor) protein, scientists typically use recombinant protein expression techniques where the MANF gene is inserted into a suitable host organism (like bacteria like E. coli) to produce large quantities of the protein, which can then be purified through various methods like cell lysis, centrifugation, and chromatography.

Key steps involved:

Gene cloning:

The MANF gene is isolated from a source (e.g., human cDNA library) and cloned into an expression vector designed for protein production in the chosen host organism.

Transformation:

The expression vector containing the MANF gene is introduced into the host cells (like E. coli).

Protein expression:

The transformed cells are cultured under optimized conditions to induce the expression of the MANF protein.

Cell lysis:

Once the protein is expressed, the cells are lysed to release the protein into a soluble fraction.

Purification:

The crude protein extract is then purified using various chromatography techniques (e.g., affinity chromatography, ion exchange chromatography, size exclusion chromatography) to isolate the MANF protein from other cellular components"

[LAJ12345 in reply to Boscoejean]

Thanks.

[Bolt_Upright in reply to Boscoejean]

I'll dig out my old chemistry set!

[Boscoejean in reply to Bolt_Upright]

I am interested in the recipes and the idea of using ecoli as part of the process makes me wonder and the part of the process where they say they use the chromatography is that supposed to give us reassurance that the ecoli is going to be purged from the remedy? You have to wonder how these plans originate.

[JayPwP]

The key word is 'Could' 😆😆