Ozone enemas as PD treatment

[kaypeeoh]

Eur J Pharmacol

, 811, 148-154 2017 Sep 15

Medical Ozone Promotes Nrf2 Phosphorylation Reducing Oxidative Stress and Pro-Inflammatory Cytokines in Multiple Sclerosis Patients

Livan Delgado-Roche 1 , Mario Riera-Romo 2 , Fernando Mesta 3 , Yanet Hernández-Matos 4 , Juan M Barrios 5 , Gregorio Martínez-Sánchez 6 , Said M Al-Dalaien 7

Affiliations Expand

PMID: 28623000 DOI: 10.1016/j.ejphar.2017.06.017

Abstract

Oxidative stress and inflammation play key roles in the pathogenesis of Multiple sclerosis (MS). Different drugs have been used in the clinical practice, however, there is not a completely effective treatment. Due to its potential therapeutic action, medical ozone represents a promising approach for neurodegenerative disorders. The aim of the present study was to address the role of ozone therapy on the cellular redox state in MS patients. Ozone (20μg/ml) was administered three times per week during a month by rectal insufflation. The effect of ozone therapy on biomarkers of oxidative stress and inflammation was addressed by spectrophotometric and immunoenzymatic assays. Furthermore, we investigated the action of ozone on CK2 expression and Nrf2 phosphorylation by western blotting analysis. Medical ozone significantly improved (P < 0.05) the activity of antioxidant enzymes and increased the levels of cellular reduced glutathione. In accordance, a significant reduction (P < 0.05) of oxidative damage on lipids and proteins was observed in ozone-treated patients. As well, the levels of pro-inflammatory cytokines TNFα and IL-1β were lower after ozone treatment. Ozone therapy incremented the CK2 expression together with Nrf2 phosphorylation in mononuclear cells of MS patients. These findings suggest that ozone´s antioxidant and anti-inflammatory effects might be partially associated with an induction of Nrf2 phosphorylation and activation. These results provide new insights on the molecular events modulated by ozone, and pointed out ozone therapy as a potential therapeutic alternative for MS patients.

Keywords: CK2 expression; Multiple sclerosis; Nrf2 phosphorylation; Oxidative stress; Ozone therapy; Pro-inflammatory cytokines.

Copyright © 2017. Published by Elsevier B.V.

[kaypeeoh]

I've used ozone in my veterinary practice for 3 years. Insufflation of ozone into the rectum is easy to do. I use a medical ozone generator that lets me choose the strength of the ozone. The article used 20 micrograms ozone per ml of oxygen. I dialed in 20 micrograms and filled an ozone bag. Then...inserted it.

[Nitro53 in reply to kaypeeoh]

Then what?

[johntPM]

For many years constipation was my worst PD symptom. I finally found a method that eliminated most of the problems: macrogel (Movicol) dispersed in yogurt/kefir every night; water enema, about every 2 days. The macrogel keeps the faeces soft; the yogurt improves the taste which I found obnoxious; the enema gives me something to push on.

[park_bear in reply to johntPM]

FWIW, I found that high-dose thiamine restored normal colon peristalsis and eliminated my constipation problem

[bepo in reply to johntPM]

You might consider a coffee enema, which stimulates the vagus nerve, which is very helpful to the liver.

[bepo]

Ozone is the new miracle cure for many chronic diseases. Go to RobertRowenMd on YouTube. He actually cured Ebola with ozone in 5 out of 5 patients. Then, suddenly, Sierra Leone, where initially they were so grateful this was a cure, kicked Dr. Rowen and other MD's out of the country? Why? Money talks.