Baicalin suppresses prostate cancer through CDK6/FOXM1 axis

[Graham49]

Published: 08 May 2020

Baicalin suppresses the cell cycle progression and proliferation of prostate cancer cells through the CDK6/FOXM1 axis

Ziqiang Yu, Changsheng Zhan, Hexi Du, Ligang Zhang, Chaozhao Liang & Li Zhang

Molecular and Cellular Biochemistry volume 469, pages169–178(2020)Cite this article

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Abstract

The aim of this study was to investigate the effects and mechanisms of baicalin against prostate cancer cell growth and cell cycle progression. A human prostate cancer cell line LNCaP was engrafted into nude mice, and the oncogenicity of LNCaP cells was analyzed. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay was used to measure LNCaP and PC3 proliferation capability. CDK6 mRNA level was detected using qRT-PCR. Western blotting was performed to assess the levels of cell cycle-associated proteins. In addition, cell cycle and apoptosis were analyzed using flow cytometry. Baicalin significantly decreased the expression of cell cycle-associated proteins. Furthermore, baicalin showed an observable effect on proliferation, cell cycle progression and apoptosis in LNCaP and PC3 cells. Upregulation of CDK6 and FOXM1 reversed the effect of baicalin. CDK6- and FOXM1-mediated cell growth attenuated the protective effect of baicalin in prostate cancer. This study presented an understanding of the role and mechanism of baicalin in prostate cancer cells, providing a new target and therapies for the prevention and treatment of prostate cancer.

[henukit]

Good. I got stocked on it prior to starting Provenge, or better to attempt to start. The idea is that it helps to amplify immune effect, pushing closer to the abscopal effect. I will try to resume taking it, although don't know BBB penetration which is important in my case.

[rassusukumaran in reply to henukit]

What kind of dosage are you thinking about?

[henukit in reply to rassusukumaran]

Based on limited trials doses 200-2800mg were found safe and produced detectable levels in blood. BBB penetration is also noted. Oral bioavaillability is about 13%. The therapeutic doses are not established. Wild guess.