Diagnostic value of SHB expression in human prostatecancer tissue and in benign prostatic hyperplasia

[puxi]

M. Schmidt1

, E. Svobodova2

, V. Semanekova2

, M. Klabanova3

, J.

Mares2

1

Urology Clinic, 2nd Medical Faculty, Prague, Czech

Republic, 2

Institute of Biology and Medical Genetics, 2nd

Medical Faculty, Prague, Czech Republic, 3

Diana Lucina,

Prague, Czech Republic

Abstracts from the 51st European Society of Human Genetics Conference: Posters 465

The general diagnosis for metastatic CaP remains poor and

locally advanced disease is difficult to treat successfully.

Except the PSA, no specific diagnostic test for CaP is

currently available. SHB protein takes part in regulation

system of apoptosis, angiogenesis and cell cycle. Reduced

tumour growth in vivo and increased c-Abl activity in PC3

prostate cancer cells overexpressing SHB was described in

mice. In this first-ever study evaluating SHB expression in

human prostate, transcription levels of SHB in prostate

cancer and benign prostate hyperplasia was compared.

Isolation of total RNA from prostate tissue in 127 patients

with histologically proved prostate cancer and 55 patients

with benign prostate hyperplasia was performed. After

qRT-PCR, comparisons of transcriptional levels in prostate

cancer and BPH and in prostate cancer groups classified by

staging, Gleason score, age and PSA were evaluated.

Underexpression of SHB in prostate cancer tissue comparing to benign prostate hyperplasia (p< 0.001) and decreased

expression in locally advanced disease (T2 v.s. T3-4)

(p = 0.0066) were detected. There were no differences in

comparison of the groups distributed by Gleason score, age

and PSA. SHB was underexpressed in prostate cancer tissue

compared to BPH and its expression was lower in locally

advanced tumours. This data may be used to make the

diagnosis of prostate cancer more precise. Research was

supported by Diana Lucina, GAUK 200 090 and IG

00064203.

M. Schmidt: None. E. Svobodova: None. V. Semanekova: None. M. Klabanova: None. J. Mares: None.

[NPfisherman]

Thanks for posting, Puxi...

Fish

[j-o-h-n]

All Czeched Thanks....

Good Luck, Good Health and Good Humor.

j-o-h-n Friday 07/12/2019 6:35 PM DST