Berberine

[Graham49]

This paper suggests that high levels of Berberine may be counterproductive.

Anticancer Agents Med Chem. 2018 Dec 11. doi: 10.2174/1871520619666181211121405. [Epub ahead of print]

Berberine Effects on NFκB, HIF1A and NFE2L2/AP-1 Pathways in HeLa Cells.

Belanova A1, Beseda D1, Chmykhalo V1, Stepanova A1, Belousova M2, Khrenkova V3, Gavalas N4, Zolotukhin P1.

Author information

Abstract

BACKGROUND:

Berberine has multitudinous anti-cancer stem cells effects making it a highly promising candidate substance for the next-generation cancer therapy. However, berberine modes of action predispose it to significant side-effects that probably limit its clinical testing and application.

MATERIALS AND METHOD:

HeLa cells were treated with two concentrations of berberine (30 and 100 μM) for 24 hours to asses the functioning of the NFE2L2/AP-1, NFκB and HIF1A pathways using 22 RNAs expression qPCR-based analysis.

RESULTS:

Berberine effects appeared to be highly dose-dependent, with the lower concentration being capable of suppressing the NFκB functioning and the higher concentration causing severe signaling side-effects seen in the HIF1A pathway and the NFE2L2 sub-pathways, and especially and more importantly in the AP-1 sub-pathway.

CONCLUSION:

The results of the study suggest that berberine has clinically valuable anti-NFκB effects however jeopardized by its side effects on the HIF1A and especially NFE2L2/AP-1 pathways, its therapeutic window phenomenon and its cancer type-specificity. These, however, may be ameliorated using the cocktail approach, provided there is enough data on signaling effects of Berberine.

Copyright© Bentham Science Publishers; For any queries, please email at [e-mail].

KEYWORDS:

AP-1; Berberine; HIF1A; HeLa cells; NFE2L2; NFκB; mRNA; pre-mRNA; signaling pathways

[jdm3]

Thanks. I couldn't find the paper. What was the dosing for low and high?

[Graham49 in reply to jdm3]

30 and 100 micro M for the cell line as posted in the abstract. I've no idea how this relates to a human dose. The full paper is $95 to buy.

Research Article

Berberine Effects on NFκB, HIF1A and NFE2L2/AP-1 Pathways in HeLa Cells

(E-pub Ahead of Print)

Author(s): Anna Belanova , Darya Beseda , Victor Chmykhalo , Alisa Stepanova , Mariya Belousova , Vera Khrenkova , Nikolaos Gavalas, Peter Zolotukhin*.

Journal Name: Anti-Cancer Agents in Medicinal Chemistry

DOI : 10.2174/1871520619666181211121405

[jdm3 in reply to Graham49]

Thanks. Yes, I was trying to relate to supplemental dosing in humans. I will have to figure out what 30 and 100 μM means in terms of mg.

[PhilipSZacarias in reply to Graham49]

You can probably obtain a free copy of paper from Sci-Hub. Look for active/functional sites by searching for Sci-Hub.

[Graham49 in reply to PhilipSZacarias]

Thanks Philip

[PhilipSZacarias in reply to Graham49]

You are most welcome

[cujoe]

Not that it would necessarily discount the findings of the research, it was done on the "notorious" HeLa (aka, Henriette Lacks) immortal cervical cancer cells. For those that don't know the story of these cells, here is the Wiki version and an excellent video by British documentarian Adam Curtis.

HeLa Wiki Page

en.wikipedia.org/wiki/HeLa

The Way of All Flesh by Adam Curtis

thoughtmaybe.com/the-way-of...

Be Well - cujoe

[Graham49 in reply to cujoe]

Thanks cujoe. Very interesting.

[PhilipSZacarias]

Interesting paper. The bioavailability of Berberine is not high and therefore typical manufacturer recommended dosages on the bottle should not be a problem. Phil

[j-o-h-n]

Anyway it rhymes:

youtube.com/watch?v=zdistoU...(Not working as expected?)

Good Luck, Good Health and Good Humor.

j-o-h-n Sunday 03/03/2019 11:42 AM EST

[Kuanyin]

This is the problem with many of these research papers: they use blood volume measurements but most of us deal with mg and grams. Good luck trying to convert one to the other: as far as I know there are no such conversion charts or tables. 24 hours is the length of the experiment!? Lastly, does anyone know to what the "cocktail approach" mentioned in the conclusion actually refers to? IMO, unless I have missed something, this study is useless. Reads like something the researchers did on a lunch hour break.