How does the art laboratory op...

[sarth03]

How does the art laboratory operate and what safety measures are taken? What will happen to the eggs and sperm after retrieval?

[sili009]

On the day before the oocyte (egg) retrieval, oocyte culture dishes are labeled with the patient’s name, date of birth, and ART identification number. The number and size of the ovarian follicles determines the number of dishes prepared (one dish for each large follicle). The dishes are filled with a rinsing fluid in the outer well for removing blood and other extraneous cells from the egg, and a nutrient fluid for the culture of the egg is in the center well.

The dishes are then placed in the embryology incubator for warming to body temperature. The incubator also adjusts the pH of the culture media to the human body. A large amount of rinsing media is equilibrated in the incubator. Media is also equilibrated in the andrology laboratory for processing of the sperm.

One of the more important and useful IVF laboratory instruments is the mobile IVF chamber. It is a pediatric isolette (incubator) on wheels that has been modified to contain a scanning microscope. Prior to the egg retrieval it is warmed to body temperature and put at the normal neutral pH of the culture dish media. These conditions avoid any abrupt changes the eggs might encounter outside the body. The chamber has two small doors on each side. The embryologists place their hands on one side to identify eggs and move dishes. The OR nurse uses the opposite doors to pass test tubes containing the follicular fluid to the embryologist.

Upon arrival in the operating room the patient’s identity is verified by the embryologist, physician, and safety nurse. They also verify that the culture dishes are correctly labeled. At the time of surgery the mobile chamber is loaded with the equilibrated petri dishes for receiving the follicular fluids.

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take care and don't take tension everything will gonna be alright have faith

[sarth03 in reply to sili009]

thanx sili09