Serrapeptase...

[JANVAN]

Hi everybody .........just seen some videos (one from Dr. Eric Berg) about

serrapeptase. I know, scientific evidence (via trials) is not really strong...,

but have someone used it already ?? When you consider the biochemical pathway,

and take it on an empty stomach, it could be help a bit, I suppose.....??

[park_bear]

Serrapeptase is a big molecule and there is a real question as to whether it is actually absorbed intact. I use it in my toothpaste formula because it breaks up bacterial biofilms.

[Rhyothemis in reply to park_bear]

Nattokinase, also a fibrinolytic enzyme, has been found to be bioavailable when taken orally:

ncbi.nlm.nih.gov/pubmed/?te...

So perhaps ...

[park_bear in reply to Rhyothemis]

Nattokinase has a molecular weight of about 500. Serrapeptase weighs in at about 100 times that - a vastly larger molecule. So they are not comparable.

Edit - Preceding retracted due to being in error

Edit2 - Confirmed that the variety of nattokinase used in the above study is the high molecular weight variety.

[Rhyothemis in reply to park_bear]

Serrapeptase has a molecular weight of 52 kDa:

sciencedirect.com/science/a...

Nattokinase has a molecular weight of 27.7 kDa:

sciencedirect.com/science/a...

Lumbrokinase proteins have molecular weights ranging from of 33.6 to 54.7 kDa and can be absorbed through the intestine of rats:

ncbi.nlm.nih.gov/pmc/articl...

"Gastrointestinal absorption of macromolecules, such as, proteins can be explained by three mechanisms as follows [16,17].

First, proteins are absorbed via receptor mediated endocytosis are as

some growth factors. Second, proteins are absorbed by pinocytic

vesicles, which are formed by the brush border membrane of the

intestine, and normally fuse with lysosomes to form phagolysomes. Only

if a protein escapes hydrolysis can it conceivably enter the

extracellular space and reach the blood stream. Third, protein

absorption by trans-epithelial transport via the M cells of Peyer's

patches could be involved. Foreign proteins, such as, lumbrokinase are

unlikely to be absorbed via receptor-mediated endocytosis, and thus, the

intestinal absorption of lumbrokinase is more likely to involve the

second and third mechanisms. Because it is a complex of strong serine

proteases, lumbrokinase is resistant to degradation by some cellular

enzymes, and thus, it could be transferred intact and across the cell

membrane by pinocytic vesicles or epithelial cells (M cells)."

~

It is not clear to me how much size matters if either of the latter two transport mechanisms are involved; Fig 2 from the article shows absorption across the 33.6 - 54.7 kDa range.

[park_bear in reply to Rhyothemis]

Very interesting. I believe you are correct which would mean this reference is incorrect:

chemsrc.com/en/cas/133876-9...

[Hidden in reply to park_bear]

Is this reference also incorrect? Very confusing!

pubchem.ncbi.nlm.nih.gov/co...

Art

[park_bear in reply to Hidden]

Same as the reference that I cited. Yet the reference cited by Rhyothemis and Wikipedia have it as a big 27 kDa molecule.

[Rhyothemis in reply to Hidden]

This sounds like a question for Quora (they have chemists)... I will ask.

~

Edit 4/5/2019:

Here is the link to my Quora question, after ~24 hrs, no response:

qr.ae/TW1xFW

Perhaps I could ask PubChem?

[Rhyothemis in reply to Hidden]

Here's the response from PubChem:

Dear Colleague,

Thank you for reporting the error. This is due to faulty

user-supplied synonyms - the displayed name should not be nattokinase.

I've notified the developers and they should be able to implement a fix

in a few business days.

Best regards,

User Services

NCBI | NLM | NIH

~

I'm impressed by the very quick response. Great customer service for something that's free.

[Hidden in reply to Rhyothemis]

I am a fan of nattokinase! It has multiple health values and a good safety profile!

healthunlocked.com/parkinso...

ncbi.nlm.nih.gov/pmc/articl...

Art

[JANVAN in reply to Hidden]

But is there some evidence for PD ?? I don't have all the other issues (heart o.k., blood pressure o.k., no obesitas, no cholesterol,...)

So, how much will I benefit of it ? And if I take it, is there some danger of my blood getting to thin ??

[Hidden in reply to JANVAN]

Yes, there is the potential for blood thinning, so you would definitely never want to take it with any other type of blood thinner, even the natural ones like fish oil, vitamine and aspirin to name just three and given the health conditions it is used for, definitely under your doctors approval and supervision.

Art