Cannabidiol (CBD).: A new cell study... - Advanced Prostate...

Advanced Prostate Cancer

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Cannabidiol (CBD).

pjoshea13 profile image
pjoshea13

A new cell study below, for CBD users. Seems to be everywhere these days.

"Cannabidiol (CBD), a phytocannabinoid derived from Cannabis sativa, has anti-inflammatory and anti-oxidant properties, and displays anti-proliferative activity."

-Patrick

ncbi.nlm.nih.gov/pubmed/301...

Front Pharmacol. 2018 Aug 13;9:889. doi: 10.3389/fphar.2018.00889. eCollection 2018.

Cannabidiol (CBD) Is a Novel Inhibitor for Exosome and Microvesicle (EMV) Release in Cancer.

Kosgodage US1, Mould R2, Henley AB2, Nunn AV2, Guy GW3, Thomas EL2, Inal JM4, Bell JD2, Lange S5,6.

Author information

1

Cellular and Molecular Immunology Research Centre, School of Human Sciences, London Metropolitan University, London, United Kingdom.

2

Research Centre for Optimal Health, Department of Life Sciences, University of Westminster, London, United Kingdom.

3

GW Research, Sovereign House Vision Park, Cambridge, United Kingdom.

4

School of Life and Medical Sciences, University of Hertfordshire, Hatfield, United Kingdom.

5

Tissue Architecture and Regeneration Research Group, Department of Biomedical Sciences, University of Westminster, London, United Kingdom.

6

Department of Pharmacology, University College London School of Pharmacy, London, United Kingdom.

Abstract

Exosomes and microvesicles (EMV) are lipid bilayer-enclosed structures, released by cells and involved in intercellular communication through transfer of proteins and genetic material. EMV release is also associated with various pathologies, including cancer, where increased EMV release is amongst other associated with chemo-resistance and active transfer of pro-oncogenic factors. Recent studies show that EMV-inhibiting agents can sensitize cancer cells to chemotherapeutic agents and reduce cancer growth in vivo. Cannabidiol (CBD), a phytocannabinoid derived from Cannabis sativa, has anti-inflammatory and anti-oxidant properties, and displays anti-proliferative activity. Here we report a novel role for CBD as a potent inhibitor of EMV release from three cancer cell lines: prostate cancer (PC3), hepatocellular carcinoma (HEPG2) and breast adenocarcinoma (MDA-MB-231). CBD significantly reduced exosome release in all three cancer cell lines, and also significantly, albeit more variably, inhibited microvesicle release. The EMV modulating effects of CBD were found to be dose dependent (1 and 5 μM) and cancer cell type specific. Moreover, we provide evidence that this may be associated with changes in mitochondrial function, including modulation of STAT3 and prohibitin expression, and that CBD can be used to sensitize cancer cells to chemotherapy. We suggest that the known anti-cancer effects of CBD may partly be due to the regulatory effects on EMV biogenesis, and thus CBD poses as a novel and safe modulator of EMV-mediated pathological events.

KEYWORDS:

cancer; cannabidiol (CBD); combinatory treatment; exosomes; inflammation; microvesicles (MVs); mitochondria; peptidylarginine deiminase (PAD)

PMID: 30150937 PMCID: PMC6099119 DOI: 10.3389/fphar.2018.00889

20 Replies

I find all this cannabinoid info appealing, but Dr. Myers observation that in his patient population it's use was associated with improved growth of prostate cancer tumors makes me reluctant to adopt it's use until I am in hospice.

BigRich profile image
BigRich in reply to cesanon

Did Dr. Myers tell you this or did you read this observation? If read, what is the link; so that, I can read it and judge its validity.

Rich

cesanon profile image
cesanon in reply to BigRich

He told me face to face in an office visit.

He expressed some irritation with those of his patients who continued to vigorously commit to it's use disregarding his advice and in the face of their own aggressive PSA trajectories.

I don't believe he ever addressed the issue one way or another in any of his videos or articles.

BigRich profile image
BigRich in reply to cesanon

Thank you for your warning. I appreciate it.

Rich

curt504a profile image
curt504a in reply to cesanon

Only 2nd hand reporting; we like going to medical conferences, 2 medical cannabis conferences this year, all PHD/ MDs presenters, Patients out of time the best so far, no reports of non-beneficial use of full spectrum CBD especially if with THC. BTW newer products available in the "legal states" now include THCa (acid form) which is not psycho active.

Miller's observational error may have been from non-compliant patients not following other best practices at the same time and the poor outcomes where from their poor practices and not cannabis (my guessing).

Poor medical outcome meta data studies fail to consider other issues in play that where the actual cause of the negative outcome... But only bringing this up but not suggesting this WAS the case for Miller's views, only a possibility.

Cannabis use is the wild west right now. Little standardization, little accurate labeling even. Worse per the conferences, product efficacy vary from one brand, from same brand batch to batch. Much like vintages of wine vary widely year to year, so can cannabis with same brand and certainly between different brands. But consider that batch to batch can vary efficacy as well least until we have better lab testing, better manufacturing processes and much better plant products going into the end resulting products.

One must experiment AND use a cannabis Practitioner in the legal states who know the local producers and their efficacy to get a treatment plan based on the actual products on the shelves NOW. And efficacy varies over time, forcing shifting from one product to another. One needs to find the cannabis practitioners in the legal states.

The 50 state legal CBD oils from hemp have low efficacy for cancer, some.... but not as high as the best vendors and ratios that contain THC from the legal states.

Best of luck all.

pjoshea13 profile image
pjoshea13 in reply to curt504a

Thanks Curt. -Patrick

curt504a profile image
curt504a in reply to curt504a

At the Patients Out Of time (cancer and PTSD and pain focused) conference we met this cannabis Dr who is very talented will do phone consults for $300 to anyone anywhere. He's in Main. but can help you with a treatment plan for your cancer type / issues and help with what to buy in what state. Then its a car ride or plane trip (as we do).

Dr Dustin Sulak / healer.com

Hidden profile image
Hidden in reply to curt504a

I concur compadre!

IT,

There is another possibility: detection bias - caused by men who feel they are running out of road trying things they might have once rejected.

-Patrick

Hidden profile image
Hidden in reply to pjoshea13

I smoked as a teen and turned it off for 40 yrs until dx. I didn’t use during my quest for money because I felt it would slow me down ... now , you are correct ,I’ve already been slowed down with APC.. now I appreciate all of the high and conscience raising qualities that reduce my gripes and allow me to enjoy daily life a little bit more than without... Am I running out of road? Only god knows... Does any form of canaby help? I believe they all do. Does canaby stop cancer. I pray so. I’m on no pharma besides ADT. That’s enough for me. I could be on Prozac as offered by a cancer shrink . My wife is adamant about no more chemicals.. Full strenght THC ,CBD = RSO oil for me daily for three years keeps me mellow and out of the phycotropic pill line for life. Take care

WSOPeddie profile image
WSOPeddie in reply to Hidden

I don't indulge very often. Can't do it if there's anything on the agenda that would require me to drive. If I take it in the evening my dreaming gets so animated that I have trouble sleeping, which sounds illogical, I know. I'm still awake but dreaming as soon as I shut my eyes, or is that hallucinating? Maybe I should try just the CBD oil. BTW, that Moose and Lobsta strain is top shelf.

I have to say I've been using since I was 14 and I'm now 60. While I do have the Cancer and it's a Gleason 9, I've only had ~2cm growth in 4 lymph nodes, while I have been metastatic since 2009. I'm not saying my use has had an influence, and I wouldn't rule it out either. I have followed all protocols as prescribed. I'm currently on the Lupron + Zytiga + Prednasone.

While I am not a biochemist, or doctor of any sort, there aren't any of them here in the states who can say a damn thing about Cannabis since no one can study it here. There are no empirical studies to site. We have only anecdotal evidence.

I have over 46 years experience. It is not addictive. I have traveled a lot for business and never bring it with me, and never feel any ill effects.

My Rant below:

Until it is removed from the schedule 1 drugs by the DEA, and the world wakes up to realize that this plant has so many uses, not just medicinal, will we see the honest research to see what combination of CBD and THC is optimal as a medicine, and for what diseases. It has only ever been made illegal to help the rich get richer, and as a way to control minority populations, and been damn fine for that.

Genesis 1:29:God said, “Behold, I have given you every seed-bearing plant on the face of all the earth, and every tree whose fruit contains seed. They will be yours for food.

Peace,

Bees

Kevinski65 profile image
Kevinski65 in reply to zenbee13

Zeebee13: I heard that smoking pot accelerated prostate cancer while eating it, Indica only, slows it down. What are your thoughts on this? I too am I Gleason 9, doubling every 5 months, M1, 3 bone mets, osteopenia. I've was on Lupron for 2.5 and then Xtandi was added. Presently have a .02 PSA and vanishing bone mets. I've taken Zyflamend. I've toyed with the idea of cannabis but an undure how to approach it. Could you advise?

Hidden profile image
Hidden in reply to Kevinski65

It’s worth a try. What do you have to lose? Except “ C” anxiety and stress. My personal thought is that if it does not kill pc ,it surely helps me with the side effects and anxiety due to APC and treatment.. If I didn’t use canaby I’d be more of a mess right now.. currently I’m in a better space than anytime during my last 3yrs after dx. I am a M.M. Proponent along with half of the People .. the other half drink and uses prescription drugs.... any social stigma about pot use is archaic and outdated... new meds coming out ..we need to make it until some big break comes in cancer treatment... enjoy life!

zenbee13 profile image
zenbee13 in reply to Kevinski65

Hi Kev

My answer is that I don’t know. I can tell you that for me it has helped to deal with pain and side effects. Beyond that because we cannot get good testing done due to its federal status we are all blind to possible improvements that might be brought to many lives.

So I do what feels right to Me. What else are we left with?

Peace

Bees

Hidden profile image
Hidden in reply to zenbee13

Amen! Your are Zen!

youtube.com/watch?v=W_4R43_...

Good Luck and Good Health.

j-o-h-n Thursday 08/28/2018 9:09 PM EDT

WSOPeddie profile image
WSOPeddie in reply to j-o-h-n

j-o-h-n, I thought this is where you were going:

youtube.com/watch?v=aYHDzrd...

j-o-h-n profile image
j-o-h-n in reply to WSOPeddie

Great Movie.. Thanks...

youtube.com/watch?v=A6c6eUe...

Good Luck and Good Health.

j-o-h-n Monday 09/03/2018 2:07 AM EDT

Myers could have been referring to either CBD and or THC. I didn't drill down.

I did get the impression that the patients he was talking about were patients In which cannabis was Incorporated into at a fundamental level Into their belief system.

So I assume someone with an ocd orientation around cannabis is taking it all, in more than medicinal levels.

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