Curcumin suppresses proliferation of human PCa stem cells.

[pjoshea13]

New cell & mouse study below.

"Many studies have demonstrated that curcumin can effectively inhibit the proliferation, invasion, and tumorigenesis of prostate cancer cells in vitro and in vivo. In this study, CD44+/CD133+ human prostate cancer stem cells (HuPCaSCs) were isolated from the prostate cancer cell lines Du145 and 22RV1. Curcumin treatment of these cells resulted in the inhibition of in vitro proliferation and invasion, and cell cycle arrest."

-Patrick

ncbi.nlm.nih.gov/pubmed/288...

Gene. 2017 Aug 23. pii: S0378-1119(17)30645-5. doi: 10.1016/j.gene.2017.08.008. [Epub ahead of print]

Curcumin suppresses proliferation and in vitro invasion of human prostate cancer stem cells by ceRNA effect of miR-145 and lncRNA-ROR.

Liu T1, Chi H2, Chen J2, Chen C2, Huang Y3, Xi H3, Xue J4, Si Y5.

Author information

Abstract

Many studies have demonstrated that curcumin can effectively inhibit the proliferation, invasion, and tumorigenesis of prostate cancer cells in vitro and in vivo. In this study, CD44+/CD133+ human prostate cancer stem cells (HuPCaSCs) were isolated from the prostate cancer cell lines Du145 and 22RV1. Curcumin treatment of these cells resulted in the inhibition of in vitro proliferation and invasion, and cell cycle arrest. The expression levels of cell cycle proteins (Ccnd1 and Cdk4) and stem cell markers (Oct4, CD44, and CD133) were decreased in curcumin-treated HuPCaSCs. Microarray analysis and northern blotting assays indicated that miR-145 was overexpressed in curcumin-treated HuPCaSCs. Insights of the mechanism of competitive endogenous RNAs (ceRNAs) were gained from bioinformatic analysis, bioinformatics analysis and luciferase activity assays showed that the lncRNA-ROR and Oct4 mRNA both contain miR-145 binding sites, and Oct4 and lncRNA-ROR directly compete for microRNA binding. Curcumin induced high miR-145 expression and inhibited the expression of lncRNA-ROR. The tumorigenicity of curcumin- treated HuPCaSCs in nude mice was significantly reduced. In summary, reducing the expression of endogenous lncRNA-ROR could effectively increase the available concentration of miR-145 in HuPCaSCs, where miR-145 prevents cell proliferation by decreasing Oct4 expression. In particular, we hypothesized that lncRNA-ROR may act as a ceRNA, effectively becoming a sink for miR-145, thereby activating the derepression of core transcription factors Oct4. Thus, curcumin suppresses the proliferation, in vitro invasion, and tumorigenicity of HuPCaSCs through ceRNA effect of miR-145 and lncRNA-ROR caused.

Copyright © 2017. Published by Elsevier B.V.

KEYWORDS:

Competitive endogenous RNAs (ceRNAs) effect; Human prostate cancer stem cells (HuPCaSCs); Long non-coding RNA-ROR (lncRNA-ROR); MicroRNA-145 (miR-145); Proliferation and invasion

PMID: 28843521 DOI: 10.1016/j.gene.2017.08.008

[BigRich]

Patrick,

I believe curcumin increases the risk of gall stones, and since I have them it orevents me from taking curcumin.

Rich

[pjoshea13 in reply to BigRich]

Rich,

I believe curcumin decreases the risk of gall stones. However, curcumin also causes gall bladder contraction, which wouldn't be pleasant if the gall bladder contained significant sized stones, I suppose.

-Patrick

[BigRich in reply to pjoshea13]

Patrick,

I would like to increase my knowledge. Please provide a link that shows curcumin decreases the risk of gall stones.

Rich

[pjoshea13]

Rich,

Mouse study:

ncbi.nlm.nih.gov/pubmed/?te...

ayurvediccure.com/5-easy-ho...

No human trials.

-Patrick

[BigRich in reply to pjoshea13]

Thank you. It is human to err, and divine to forgive. You were correct.

Rich

[BigRich]

Thank you Nalakrats. I will keep Zyflamend since it was recommended to me 13 yers ago by Dr. Arron Katz' my cryosurgeon. He has done research on it and its intetraction with PCa.

Rich

[enzo1]

Are you sure about the content of Curcuminin Zyflamend? Unfortunately I'm not enough good in english, but I've read that you have to take about 1200 mg by day of curcumin 95%. Also a doctor, here, prescribed me thatamount, about 600 mg 2 times/day. On the label of Zyflamend they write: 10 mg Turmeric (Curcuma longa) rhizome....

Enzo

[ctarleton in reply to enzo1]

Enzo,

Questo strumento di traduzione potrebbe aiutare a leggere e scrivere le cose qui.

This translation tool could help you read and write things here.

translate.google.com/

Charles

[enzo1 in reply to ctarleton]

Thank you, I know google translate, but it gives its best translating formal language, "school english". The "google translate" of what you write here, sometimes, is comic, in italian. I can understand most of what you write, but it isn't always enough. I think it could be the same if you tried to undestand and translate me and my friend writing about bike riding. I'm from a valley in north Italy, my wife comes from a small town just 70 km away. If my father speaks in dialect with his brother, she understands 30%.

... but what I understand here, is very useful, thanks to you.

Enzo

[pjoshea13]

"Turmeric contains approximately 2% curcumin"

lipotherapeia.com/the-peach...

-Patrick

[softwaremom00]

I thought I would add my Curcumin supplement to the list. My honey(with PCa) is taking this and when I have aches and pains I take it and it really helps. It is pricey though.

Bosmeric-SR - bosmeric-sr.com - an integrative Dr in town suggested it.

It might help someone out there who is not having success with other curcumin supplements.

[pjoshea13 in reply to softwaremom00]

Curcumin is not very bioavailable, & the inclusion of Bioperine is a clue that this curcumin needs help.

Consider Longvida:

ncbi.nlm.nih.gov/pubmed/252...

If it can cross the blood-brain barrier, it is bioavailable.

Sold as CurcuBrain:

swansonvitamins.com/now-foo...

-Patrick

[softwaremom00 in reply to pjoshea13]

Thanks Patrick, I will get some and try it.

Do you know of a good Beta Glucan ?

[pjoshea13 in reply to softwaremom00]

I have used a few products over the years. However, when it comes to a mushroom product for PCa, the evidence points to turkey tail (Coriolus versicolor) as being the most likely to help.

I would like to buy an American product, but none pass the smell test - & some are expensive. So, grudgingly, I shop abroad - ORIVeDA is a Dutch company. This is what I use:

oriveda.com/e-coriolus_psp.php

Label claims at least 55% polysaccharides, at least 29% of which are 1,3/1,6 beta-D-glucans.

Can be ordered via Amazon:

amazon.com/ORIVeDA-PSP-50-e...

-Patrick

[softwaremom00 in reply to pjoshea13]

Thank you! The PSP 50 looks like a good product. We have taken the Transfer Point Beta Glucan. It seems like the price is comparable to the PSP 50. I think it is good to try different products. I will get some of the turkey tail and have him try that for a while.

How many of these a day do you recommend ? With food or without food ?

Thanks again!