Acetyl -L-carnitine

[Graham49]

Prevention, Early Detection, and Interception

Abstract 5086: Acetyl-L-carnitine (ALCAR) inhibits angiogenesis, migration and macrophage recruitment in prostatic cancer cells

Adriana Albini, Antonino Bruno, Denisa Baci, Matteo Gallazzi and Matilde Tramacere

DOI: 10.1158/1538-7445.AM2019-5086 Published July 2019

ArticleInfo & Metrics

Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA

Abstract

Many efforts have been addressed on the identification of novel active compounds from natural sources, endowed with anti-proliferative, anti-oxidant, chemopreventive properties, and their ability to target the tumour microenvironment (TME). Through metabolomics approaches we previously found that in serum samples from prostate cancer (PCa) patients, three carnitine family members were significantly decreased, suggesting a potential protective role of carnitine against PCa. Acetyl-L-carnitine(ALCAR) is an aceticacid ester of carnitine with high bioavailability and is involved in the transportof fatty acids across the inner mitochondrial membrane. We have showed that ALCAR was able reduce angiogenesis in vitro, acting on the VEGF/VEGFR2 and CXCR4/CXCL12 axes. We also found that ALCAR inhibits inflammatory angiogenesis in vivo, by reducing endothelial cells and macrophage recruitment in the matrigel plugs. Here we investigated the ability of ALCAR to interfere with key functional steps of prostate carcinogenesis and identified the molecular mechanisms involved. The effects of ALCAR on PCa cells were investigated in vitro by functional assays (adhesion, migration and invasion assays), molecular and biochemical approaches (RT-PCR, FACS, Byoplex and western blot). We found that ALCAR reduces apoptosis on PCa cells (PC3, Du-145, LNcap) and inhibits crucial tumorigenic steps such as adhesion, migration and invasion. We than confirmed the results from functional assays at molecular levels, and we found that ALCAR blocks CXCR4/CXCL12 axes, a key regulator of malignant migratory/aggressive phenotype. In accordance with our published paper, we confirmed the anti-angiogenic and ant-inflammatory properties of ALCAR, that we found to lower VEGF and CXCL8 production in PCa cell lines. Furthermore, we found a significantly reduced expression of inflammatory-related chemokines/cytokines involved macrophage recruitment such as CCL2, IL-6 and TNFα in PCa cells. Our results highlight the angio/chemopreventive and anti-inflammatory properties of ALCAR and allow the identification of multiple and overlapping mechanisms of action through which ALCAR inhibits PCa progression / metastasis. Our findings provide the rational for the employment of ALCAR, as a possible supplement for approaches of chemoprevention in subjects at high risk to develop cancer.

Citation Format: Adriana Albini, Antonino Bruno, Denisa Baci, Matteo Gallazzi, Matilde Tramacere. Acetyl-L-carnitine (ALCAR) inhibits angiogenesis, migration and macrophage recruitment in prostatic cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 5086.

©2019 American Association for Cancer Research.

[cesces]

"inhibits angiogenesis"

Strangely enough, Dr. Myers had me on small dose Viagra to increase circulation/oxygenation to the prostate cancer cells.

[henukit in reply to cesces]

As I understand, angiogenesis is partially attributed to the response of tumor microenvironment to hypoxia. Thus, reducing hypoxia through oxygenation makes sense.

[Shanti1 in reply to cesces]

Increasing circulation could also increase delivery of cancer meds, depending on what you were taking.

[Hidden]

I was advised to take acetyl L-carnitine as something to improve circulation to the penis which could aid in nocturnal erections and thus contribute to the health of the little guy. Maybe I'll increase the amount I am taking.

[TFBUNDY in reply to Hidden]

I just bought a bag of this stuff on the basis of anti angiogenesis to add to my ever increasing list of things to remember to take. Did Percy respond in any way to your increased dose ?

[Hidden in reply to TFBUNDY]

He was already pretty spry on just one capsule at night.

[TFBUNDY in reply to Hidden]

I'm pretty good but always at the Wrong Time. At 3am I need sleep. It gets so much pressure I can feel the prostate scarring pulling (ex HIFU) as it stretches out I . I just wish it performed as well when I have something to do with it except make a tent... I'm not on ADT so my testosterone levels are good. And my wife is Very Pretty..... I don't know what to do with the miserable little shit. My own dick has turned against me lol... It's like a vampire... Only comes out at night....

[Hidden in reply to TFBUNDY]

I had HIFU too but not that problem with scarring there. I had another post on peyronies problem which also involves scar tissue / plaques causing a downward bend which made things very difficult. Try adding iodoral 12.5 mg (half tablets enough) along with carnitine and add L-arginine. That combination cured my peyronies in a matter of weeks maybe it will help with your issue. L-arginine is considered the poor man's viagra so give that a try too..

[TFBUNDY in reply to Hidden]

Poor man sounds right. I'll give it a go. I put a drop of 7.5% Lugol's on my arm and covered it with cling film overnight. Got a swollen arm for my trouble. I read somewhere that arginine is to be avoided.... I will revisit iodine to see if it helps with the scarring and restricted flow. You never know.

[Hidden in reply to TFBUNDY]

You probably already know that iodoral is just lugol's solution in less messy tablet form. Arginine has been touted on this site as beneficial for prostate cancer. Good luck.

[j-o-h-n]

BJ would be just fine with me....

Good Luck, Good Health and Good Humor.

J-o-h-n Friday 08/30/2019 3:43 PM DST - Pinnes

[GeorgeGlass]

But in another article it said that choline and L-Carnitine raised TMAO. TMAO is bad for the arteries. Have you read about that?